Genome Editing by Electroporation of Cas9 Protein (GEEP) method is a method of introducing genome editing tools such as Cas9 protein and gRNA into fertilized eggs by the power of electricity (electroporation). The microinjection method, which has been used to introduce genome editing tools into fertilized eggs, requires specialized equipment such as a micromanipulator and advanced technique. On the other hand, by the GEEP method, all that is required is to line up the fertilized eggs on the electrode, and it is possible to introduce a genome editing tools into multiple (20 to 200) fertilized eggs at one time.
With GEEP method, we can produce genome-editing organisms highly efficiently. Also GEEP method can perform genome editing on a large number of fertilized eggs with minimal invasiveness under identical conditions in a short time.
The GEEP method is an innovative genome-editing method that enables genome editing with higher efficiency and quality than conventional methods.